Application

Anti-APC Antibody, clone FE9 is an antibody against APC for use in Western Blotting.

General description

Adenomatous polyposis coli protein (APC) is a ubiquitous multidomain protein that has a well documented role as a tumor suppressor. The mechanism of APC-mediated tumor suppression is still an area of active investigation; however, several studies suggest that APC is a negative regulator of the Wnt signaling pathway as it downregulates β-catenin via interactions with Axin/GSK3-β complex, thereby preventing transcription of genes such as MYC that contribute to cell proliferation. Defective APC proteins contribute to the initiation and proliferation of various types of cancers, including familial adenomatous polyposis. However, other studies have shown that APC interacts with a range of other proteins such as the EB1 microtubule-binding proteins, to regulate other processes such as cell migration.

Immunogen

Linear peptide corresponding to the N-terminus of human APC.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot in SW480 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected APC in 10 µg of SW480 cell lysate.

Specificity

This antibody recognizes the N-terminus of APC.

Target description

~147 kDa observed. Uniprot describes the full length protein at ~312 kDa This antibody was shown to detect the truncated form at ~147 kDa